| pkgconfig | Compiler configuration arguments for use of Rtwobitlib |
| twobit_read | Read/write a .2bit file |
| twobit_seqlengths | Extract sequence lengths and letter counts from a .2bit file |
| twobit_seqstats | Extract sequence lengths and letter counts from a .2bit file |
| twobit_write | Read/write a .2bit file |